International Research Journal of Education and Technology

The present study aimed to investigate the antimicrobial properties and antioxidant activity of eugenol extracted from Piper betel leaves. Piper betel, a widely cultivated plant in tropical and subtropical regions, has been traditionally used for its medicinal properties. Eugenol, a phenolic compound found in various plants, possesses significant antimicrobial and antioxidant properties In this study, eugenol was isolated from Piper betel leaves using a solvent extraction method and subjected to antimicrobial and antioxidant assays.

The antimicrobial activity of eugenol was evaluated against a panel of bacterial and fungal strains using the disc diffusion and broth microdilution methods. Results demonstrated that eugenol exhibited significant antimicrobial effects against both Gram-positive and Gram-negative bacteria, including Staphylococcus aureus, Escherichia coli, and Candida albicans. The observed inhibition zones and minimum inhibitory concentrations indicated the potent antimicrobial activity of eugenol, suggesting its potential as a natural antimicrobial agent.

Furthermore, the antioxidant activity of eugenol was assessed using various antioxidant assays, including 2,2-diphenyl-1-picrylhydrazy! (DPPH) radical scavenging and ferric reducing antioxidant power (FRAP) assays. Eugenol displayed remarkable antioxidant activity by effectively scavenging DPPH radicals and reducing ferric ions. These findings indicate the potential of eugenol as a natural antioxidant with possible implications for oxidative stress-related diseases.

This study highlights the antimicrobial properties and antioxidant activity of eugenol extracted from Piper betel leaves. The demonstrated antimicrobial effects against bacterial and fungal pathogens, as well as potent antioxidant activity, suggest the potential use of cugenol as a natural alternative for combating microbial infections and oxidative stress-related disorders. Furtlier studies are warranted to explore the underlying mechanisms of action and potential therapeutic applications of eugenol in the field of medicine and pharmaceutical sciences